Biotinylated mouse tim4-fc

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August undefined, 2024

WebTIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by … WebMar 27, 2005 · The newly identified TIM family of proteins is associated with regulation of T helper type 1 (TH1) and TH2 immune responses. TIM-1 is genetically linked to asthma and is a receptor for hepatitis A ...

Biotin anti-mouse IgM - BioLegend

WebTo produce an mAb against Tim4, Armenian hamsters were immunized with Tim4-Fc, in which the extracellular region of mouse Tim4 was joined to human IgG Fc region (11). Lymphocytes from the immunized hamsters were fused with a mouse myeloma NSObcl2 (17). One hybridoma (Mat4-9-5) that was suitable for immunohistochemical staining, WebTim4 alone did not support efferocytosis, but enhanced TAM-dependent efferocytosis. Resident peritoneal macrophages, Kupffer cells, and CD169+ skin macrophages … duwayne dalen attorney iowa

TIM-4 is the ligand for TIM-1, and the TIM-1–TIM-4 interaction ...

WebMouse Ig cocktail. Formulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation. The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C. WebTim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 … WebTim-4 can isolate extracellular vesicles from different species (human, mouse, rat, etc.) containing phosphatidylserine (PS) at their surface. Biological Activity Measured by its … duwatnum part of body

Identification of Tim4 as a phosphatidylserine receptor

Mouse CD45 Biotinylated Antibody BAM114: R&D Systems

WebRecombinant Mouse TIM-4 (Catalog # 2826-TI) inhibits anti-CD3-induced proliferation of stimulated human T cells. Human T cells cultured for 72 hours with PHA are incubated … WebMouse TIM4-Ig fusion protein Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide ... FC - Quality tested. Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per ... dusit hospitality servicesWebBackground: IgG1. R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. dusing sofa

"WebMouse TIM4-Ig fusion protein Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. ... FC - Quality tested. Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg ... " - Biotinylated mouse tim4-fc

Biotinylated mouse tim4-fc

FcRn and its role as a therapeutic target - News-Medical.net

WebFeb 9, 2024 · Tim4 is involved in recognition of MWCNTs by mouse peritoneal macrophages. (A) Recognition of MWCNTs (30 μg/mL) by WT or Tim4−/− mouse … WebThe TIM4-affinity isolation method helps overcome the disadvantages of the affinity isolation method and enables the isolation of heterogeneous SEVs at high purity. This …

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WebNov 10, 2024 · Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified) FCM-M82W5: HEK293: Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His, Avitag™ (MALS & BLI verified) FCM-M82W6 ... WebFUJIFILM biotinylated mouse tim4 fc Biotinylated Mouse Tim4 Fc, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed …

WebMar 15, 2024 · Then concentrated CM were incubated with Streptavidin Magnetic Beads (60 mg) accompanied by additional 1 μg of biotinylated mouse Tim4-Fc, 350 μL Exosome Capture Immobilizing Buffer, and 50 μL Exosome Binding Enhancer overnight at 4 °C. The next day, the beads were washed with washing buffer for three times and the bound EVs … WebRegarding Tim4 expression, cells were stained with PE-labeled cIg, or RMT4-54 (anti-Tim4: BioLegend). Mouse peritoneal resident cells were pretreated with clone 93 (anti-CD16/32: BioLegend) to block Fc receptors, and then stained with FITC-anti-CD11b (BioLegend) and the above mAbs. These cells were analyzed by an Accuri C6 cytometer (BD ...

Weblipids, including PtdCho, PtdEtn, and sphingomyelin. Tim4–Fc, a fusion protein of the extracellular region of mTim4 and hu-man IgG1–Fc (12), showed a similar specificity for PtdSer, with a three to eight times stronger affinity than that of mProS or mGas6 for PtdSer (Fig. 1). Interaction Between Mouse TAM Ligands and Their Receptors. We WebCD45 in Mouse Thymus. CD45 was detected in perfusion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD45 Biotinylated Monoclonal Antibody (Catalog # …

WebOct 24, 2007 · Mouse Tim4 is a type I membrane protein, consisting of a signal sequence and extracellular, ... Tim4-Fc-coated latex beads incubated with Tim1-Fc in the absence …

Webadjective. bio· tin· y· lat· ed ˌbī-ə-ˈti-nə-ˌlā-təd. : combined with biotin. The PCR reaction uses biotinylated primers to define a sequence of approximately 207 nucleotides within … dusit investor relationsWebMouse Tim4–Fc was prepared as described . To produce mProS and mGas6, full-length cDNAs for mProS (Gene ID: 19128) and mGas6 (Gene ID: 14456) were prepared by RT … dusit d2 the fortWebSep 23, 2016 · Nevertheless, Tim4-Fc-conjugated beads were still able to isolate sEVs efficiently from 10K sup of pMac which abundantly express … duwayne edge inglesideWebMouse Tim4–Fc was prepared as described . To produce mProS and mGas6, full-length cDNAs for mProS (Gene ID: 19128) and mGas6 (Gene ID: 14456) were prepared by RT-PCR with mRNA from mouse liver and … duwayne lambert cayce scWebMay 19, 2024 · 2.3 TIM4-Affinity Beads. 1. Mouse TIM4-Fc protein. 2. Maleimide-PEG11-Biotin: 25 mM Maleimide-PEG11-Biotin in dimethylsulfoxide (DMSO). To prepare 250 … duwayne kreager insuranceWebFeb 9, 2024 · Needle-like multi-walled carbon nanotubes (MWCNTs) are engulfed by macrophages, which cause inflammation and asbestosis-like pathogenesis in rodents; however, how macrophages recognize … duwayne mardenboroughWebTIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich … dusit thani ballroom