Ctll-2 ed50
WebThe present invention relates to cancer immunotherapy. In particular, provided herein are fusion proteins for targeting tumor associated macrophages with immunostimulatory agents. WebMeasured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.0300-0.250 ng/mL. Source. E. coli-derived human IL-2 protein Ala21-Thr153 (Cys145Ser), with and ...
Ctll-2 ed50
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WebED50 is < 10 ng/mL as determined by the dose-dependant stimulation of the proliferation of CTLL-2 cell, corresponding to a specific activity of > 1 x 10 5 IU/mg. Recommend Usage: Biological Activity ... Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. ... WebSubculture Routine. Maintain cultures between 3-9 x 10 5 cells/ml; 5% CO 2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate …
WebCorning ® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Twice per week. Some Important Considerations in Handling CTLL-2, … WebBioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL-2 cells. The ED50 is typically less than 1 ng/mL. The international units of CTGrade rh IL-15 is approximately 2.7 x 10. 5; IU/µg, which is calibrated against recombinant Human Interleukin 15 …
WebCTLL2 cells growing dependent upon the use of IL2. Actually i am agrwoing ctll2 cells and they are totally grwoing well with good cell viability. and for their culture you should do … WebED50 : 0.5 ng/mL; determined by the dose-dependen proliferation of mouse CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Purification: Size-exclusion - Gel filtration: Contains: ... Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural ...
WebBiological Activity: The ED50 as determined by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells is less than 10 ng/ml. Sequence ITCPPPMSVE HADIWVKSYS LYSRERYICN SGFKRKAGTS SLTECVLNKA TNVAHWTTPS LKCIRDPALV HQRPAPPSTV TTAGVTPQPE SLSPSGKEPA …
WebBioactivity: The ED50 of this protein, as determined by increased proliferation of mouse CTLL-2 cells stimulated with 1 ng/mL IL-2, is 0.5-1.5 µg/mL. Endotoxin: Less than 0.1 … csx statesWebMouse IL-2, amino acids Phe22-Gln169 (Accession# NM_008366), was expressed in E. coli . The 148 amino acid recombinant protein has a predicted molecular mass of 17.2 kD. The predicted N-terminal amino acid is Phe. >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis. Lyophilized, carrier-free. csx sponsorshipWebED 50 =2.5 - 15 ng/ml, corresponding to a specific activity of 0.6 - 4 x 10 5 units/mg, as determined by the dose dependent stimulation of CTLL-2 cell proliferation. Application … csx stand forWeb1.875 x 10^4. HT-2. 2.151 x 10^4. The specific activity (units/mg) of a cytokine is typically calculated using a bioassay with one set of conditions and parameters, and a defined cell line. Due to inherent variability, the specific activity measured with a different cell line, cultivated under different conditions in a different lab, can differ ... ear nose and throat michigan medicineWebThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells is ≤ 0.4 ng/ml, corresponding to a specific activity of ≥ 2.5 x 10^6 units/mg. ... natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also ... ear nose and throat mystic ctWebIL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, … ear nose and throat northampton maWebBioactivity: The ED50 of this protein, as measured by CTLL-2 cell proliferation assay, is less than or equal to 4 ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e5 Units/mg. Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 29.4 kDa. ear nose and throat milford ct