WebApr 6, 2024 · All culture media were supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics. ... For Exo treatment, the KFs were co-incubated with Exos (10 μg/μL) for 24 h, followed by the transfection of oe-NRP2/NC. After 48 h, the cells were harvested for subsequent use. ... suggesting adMSC-Exos as a candidate promising cell-free therapy … WebApr 1, 2024 · To evaluate the influence of bovine exosomes on the experiments, NTA was performed to measure the concentration of exosomes in 293T cell conditioned medium (50 mL), FBS (50 mL DMEM with 10% FBS), exo-free FBS (50 mL DMEM with 10% exo-free FBS), and exosomes that isolated from exo-free cell medium and stored in different …
The Functional Roles of RNAs Cargoes Released by Neutrophil …
WebOct 15, 2024 · For human HSCs, cells were cultured for 10-days (10% ( v/v) EXO-free FBS) and 24-h prior to EXO collection culture medium was replaced with 1% ( v/v) EXO-free FBS medium. Exosomes were isolated and purified using an EXOQuick-TC kit (System Bioscience), re-suspended in 100 μL PBS, and stored at - 80⁰C. WebApr 8, 2024 · Moreover, the protective effect of Exo-124 was abolished in mice depleted of Gr-1+ myeloid cells. It suggested that the exosomes exerted their functions by limiting the infiltration of leukocyte into the brain, thus controlling neuroinflammation following the onset of ICH. ... For the preparation of exosome-depleted fetal bovine serum (FBS ... fnf water girl mod
Angiopep-2 modified exosomes load rifampicin IJN
WebExo-FBS media supports robust growth of cells in culture equal to standard FBS media. Complete DMEM medium either with 10% standard FBS or with 10% Exo-FBS … WebJul 29, 2024 · 2. 3. Depletion of exosomes from FBS (Exosome-free FBS). Fetal bovine serum (FBS) are abundantly loaded with exosomes. FBS was converted into exosome … WebJul 29, 2024 · at 100,000 xg. FBS supernatant was collected next day and labeled as exo-free FBS. Each centrifugation step was carried out at 4°C [8]. 2. 4. Exosome isolation from conditioned medium of cell lines. Cell line was cultured in cell culture medium supplemented with Exo-free FBS (FBS depleted with exosomes). green washer and dryer set